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mtnN [2019-07-08 09:25:32]
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mtnN [2019-07-08 09:25:32]

methylthioadenosine nucleosidase
locus
BSU27270
pI
4.84
mw
25.12 kDa
protein length
231 aa Sequence Blast
gene length
696 bp Sequence Blast
function
methionine salvage
product
methylthioadenosine nucleosidase
essential
no
ec
3.2.2.9
synonyms
yrrU,mtn,mthA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]

    • Gene

    Coordinates
    2,787,130 2,787,825

    The protein

    Catalyzed reaction/ biological activity

  • S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine (according to Swiss-Prot)

    • Protein family

  • PNP/UDP phosphorylase family (with [protein|3075327DBE55C4F628171AEE248CAED5870693FA|DeoD]) (according to UniProt)

    • Structure

  • [PDB|1JYS] (from ''Escherichia coli'', 51% identity, 70% similarity) [Pubmed|11591349]

    • Expression and Regulation

    Operons

    genes
    [gene|992A24CF9492965BF0E9EEFBB6F0A06871275068|yrrT]-[gene|5B21D1E73A65B13CE4DDA32ABC71EFA54E8D1D8F|mtnN]-[gene|F195C47849F4F6B4C56E3A386F5D8C84326B01C4|mccA]-[gene|314CB4923D15140E72B0ABAFE3E8BE9CA7310E10|mccB]-[gene|177EFFDCA6E3C3BB840EED13D9F2D71F99B833B6|yrhC]
    description
    [Pubmed|17056751]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16513748], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16513748], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]
  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|12642660,16885442], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

    • regulation

  • repressed in the presence of cysteine ([protein|search|CymR]) [Pubmed|16513748]
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    Biological materials

    Mutant

  • MGNA-A850 (yrrU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/850 NBRP B. subtilis, Japan]
  • BKE27270 ([gene|5B21D1E73A65B13CE4DDA32ABC71EFA54E8D1D8F|mtnN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATTCACATTCCCTTC, downstream forward: _UP4_TAAAACGGGGAGAACGTTCT
  • BKK27270 ([gene|5B21D1E73A65B13CE4DDA32ABC71EFA54E8D1D8F|mtnN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATTCACATTCCCTTC, downstream forward: _UP4_TAAAACGGGGAGAACGTTCT

    • References

  • 17056751,16513748,12642660,15102328,16885442,24509311